SUSL Staff Directory

Dr. Kaveesha J. Wijesinghe

Position: Senior Lecturer

kwijesin@med.sab.ac.lk

Qualifications

Postdoctoral Research Associate, Cambridge University, UK (2018-2019) 

PhD in Biochemistry, University of Notre Dame, USA (2013-2018)

B.Sc. (special) Biochemistry and Molecular Biology (First Class), University of Colombo, Sri Lanka (2008-2012)


Research Interests

Molecular virology and pathogenesis of Dengue virus infection, Bio-imaging


Research Grants/Research Awards

Research Grants

UNESCO/The World Academy of Sciences (TWAS) research grant (2020-2022) 

Fellowships

National Institutes of Health (NIH) Ruth L. Kirschtein National Research Service Award from the Chemistry-Biochemistry-Biology Interface (CBBI) Program at the University of Notre Dame (2015-2017)

Awards
• ASBMB 2017 Graduate/Postdoctoral Conference Travel Award  (ASBMB- Experimental Biology-2017)

• Travel award from Graduate Student Union- Conference Presentation Grant (GSU CPG) of University of Notre Dame-2017

Research Grants

UNESCO/The World Academy of Sciences (TWAS) research grant (2020-2022) 

Fellowships

National Institutes of Health (NIH) Ruth L. Kirschtein National Research Service Award from the Chemistry-Biochemistry-Biology Interface (CBBI) Program at the University of Notre Dame (2015-2017)

Awards
• ASBMB 2017 Graduate/Postdoctoral Conference Travel Award  (ASBMB- Experimental Biology-2017)

• Travel award from Graduate Student Union- Conference Presentation Grant (GSU CPG) of University of Notre Dame-2017


Research Publication in Indexed Journals

  1. Wijesinghe, K. J. and Stahelin, R. V., 2016. Investigation of the Lipid Binding Properties of the Marburg Virus Matrix Protein VP40. Journal of virology, 90 (6), pp.3074-3085.
  2. Wijesinghe, K. J., et al., 2017. Detection of Lipid Induced Structural Changes of the Marburg Virus Matrix Protein VP40 Using Hydrogen/Deuterium Exchange Mass Spectrometry. Journal of Biological Chemistry, pp.jbc-M116.
  3. Wijesinghe, K.J., McVeigh, L., Husby, M.L., Bhattarai, N., Ma, J., Gerstman, B.S., Chapagain, P.P. and Stahelin, R.V., 2020. Mutation of Hydrophobic Residues in the C-Terminal Domain of the Marburg Virus Matrix Protein VP40 Disrupts Trafficking to the Plasma Membrane. Viruses, 12(4), p.482.
  4. Abeywickrama, C.S.*, Wijesinghe, K.J.*, Stahelin, R.V. and Pang, Y., 2019. Lysosome imaging in cancer cells by pyrene-benzothiazolium dyes: An alternative imaging approach for LAMP-1 expression based visualization methods to avoid background interference. Bioorganic chemistry, 91, p.103144.  *co-first authors
  5. Oda, S.I., Noda, T., Wijesinghe, K.J., Halfmann, P., Bornholdt, Z.A., Abelson, D.M., Armbrust, T., Stahelin, R.V., Kawaoka, Y. and Saphire, E.O., 2016 Crystal Structure of Marburg Virus VP40 Reveals a Board Basic Patch for Matrix Assembly and a Requirement of the N-Terminal Domain for Immunosuppression. Journal of virology, 90(4), pp.1839-1848.
  6. Abeywickrama, C.S., Wijesinghe, K.J., Stahelin, R.V. and Oang, Y., 2017. Bright red-emitting pyrene derivatives with a large Strokes shift for nucleus staining. Chemical Communications, 53, pp.5886-5889.
  7. Ahn, G., Kim, H., Kim, D.H., Hanh, N.H., Yoon, Y., Singaram, I., Wijesinghe, K.J., Jhonson, K.A., Zhuang, X.H., Liang, Z. and Stahelin, R.V., 2017. SH3P2 plays a crucial role at the step of membrane tubulation during cell plate formation in plants. The Plant Cell, 29(6), pp.1388-1405.
  8. Abeywickrama, C.S., Wijesinghe, K.J., Stahelin, R.V. and Pang, Y., 2019. Bright red-emitting highly reliable styryl probe with large stokes shift for visualizing mitochondria in live cells under wash-free conditions. Sensors and Actuators B: Chemical, 285, pp.76-83.
  9. Abeywickrama, C.S., Wijesinghe, K.J., Stahelin, R.V. and Pang, Y., 2019. Red-emitting pyrene–benzothiazolium: unexpected selectivity to lysosomes for real-time cell imaging without alkalinizing effect. Chemical communications, 55(24), pp.3469-3472.
  10. Abeywickrama, C.S., Wijesinghe, K.J., Plescia, C.B., Fisher, L.S., Goodson III, T., Stahelin, R.V. and Pang, Y., 2020. A pyrene-based two-photon excitable fluorescent probe to visualize nuclei in live cells. Photochemical & Photobiological Sciences.
  11. Wickramatunga, P. G. T. S., Gunawardene, Y. I. N. S., Wijesinghe, K. J., Ellepola, A. N. B., & Dassanayake, R. S. (2020). RNAi-mediated silencing of ARV1 in Setaria digitata impairs in-vitro microfilariae release, embryogenesis and adult parasite viability. Veterinary Parasitology284